Figure 1.
Chromosomal ideogram representation of the location of human DIA1 (c3orf58) and DIA1R (cXorf36).
(A) DIA1 localization on human chromosome 3 at position 3q24. (B) DIA1R localization on the human X chromosome at position Xp11.3. Gene localization is indicated by a modified red arrowhead on the right-hand side, and a light-grey line extending through the text on the left-hand side.
Table 1.
Accession numbers and identifiers for human DIA1 and DIA1R.
Figure 2.
Amino acid sequence comparison of human DIA1 and human DIA1R.
The sequence alignment was generated using CLUSTALX [164]. Identical amino acids are highlighted in red font and indicated below the alignment with an asterisk (*). Strongly similar amino acids are highlighted in green font and indicated below the alignment with a colon (:). Weakly similar amino acids are highlighted in blue font and indicated below the alignment with a full stop (.). Dissimilar amino acids are in black font. Amino acid numbering is provided above the alignment. Human DIA1 is 430, and DIA1R 433, amino acids in their immature forms (before signal peptide cleavage). Gaps required for optimal alignment are indicated by dashes. The two proteins have 28% identical, 21% strongly similar, and 13% weakly similar, residues. Standard single-letter amino acid abbreviations are used.
Figure 3.
Prediction of signal peptides in human DIA1 and DIA1R.
The amino acid sequence of (A) human DIA1 or (B) human DIA1R, was evaluated for amino-terminal signal peptides using the hidden Markov model of SignalP v3.0 [72]. C-region (cleavage-recognition region) scores are in aqua, H-region (hydrophobic region) scores are in dark blue, N-region (N-terminal signal peptide sequence) scores are in green, and the cleavage probability (that a given amino acid is the first in the mature protein) indicated in red. The significance cut-off value for all probability scores is 0.5, and is indicated by a blue dotted line. Standard single-letter amino acid abbreviations and numbering is provided below the graph.
Table 2.
Expressed sequenced tag (EST) profiles showing DIA1 gene expression in human tissues.
Table 3.
EST profiles of DIA1R gene expression in human tissues.
Figure 4.
Microarray gene expression data for DIA1 and DIA1R.
Graphical presentation of microarray expression data for (A) DIA1 or (B) DIA1R in normal, healthy human tissues. The intensity values (shown on the root-scale y-axis) are average values, where variation in the range of measurements is represented by a white box above the coloured minimal-measurement bar. Tissues are grouped according to their origin and the groups coloured: red = immune system; green = nervous system; yellow = muscle; blue = secretory glands; pink = ‘other’. Tissue abbreviation (on the x-axis) legend: BMR = bone marrow; SPL = spleen; TMS = thymus; BRN = brain; SPC = spinal cord; HRT = heart; MSL = skeletal muscle; LVR = liver; PNC = pancreas; PST = prostate; KDN = kidney; LNG = lung. Data were obtained from the GeneNote database of human genes at the Weizmann Institute [75], and are MAS5.0 normalized data obtained using the Affymetrix HG-U95 set A–E.
Table 4.
EST profiles of DIA1 gene expression during human development.
Table 5.
EST profiles of DIA1R gene expression during human development.