Table 1.
Agreement and taxonomic accuracy in regional and whole-fauna clustering at 3%.
Figure 1.
Chronogram of cox1 sequences for the Australian diving beetle fauna based on ML branch lengths which were made ultrametric with a relaxed molecular clock.
Focal clades are Hydroporini (blue, polyphyletic); Neobidessodes (green) and Exocelina (orange). Three pink clades contain paraphyletic species. Dots denote speciation events as inferred from morphospecies identification or combined genetic and morphological data. Note: Multiple dots within the pink clades omitted for clarity.
Figure 2.
Agreement and taxonomic accuracy of molecular clusters estimated with different thresholds of DNA sequence divergence and the GMYC algorithm.
Blue lines, agreement derived from clustering (percentage of clusters relative to number of morphological species used in the particular dataset), and orange lines, taxonomic accuracy of clusters (number of clusters containing all sequences of a named species and only those). (A) Full dataset and two modifications thereof (315 species: dashed lines; 260 species: dotted; 242 species: solid lines); (B) all Hydroporini; (C) Hydroporini: Sternopriscus; (D) Hydroporini: Tiporus; (E) Hydroporini: Megaporus; (F) Copelatinae: Exocelina, purple and green – agreement and taxonomic accuracy for the raw dataset, blue and orange – taxonomically revised dataset. Circles - agreement for GMYC entities, triangles – taxonomic accuracy of GMYC entities (in F, purple and green GMYC for taxonomically raw Exocelina dataset).
Figure 3.
Agreement and taxonomic accuracy at different thresholds of genetic DNA distance clustering using regional subsampling (single states and pairwise comparisons), and number of species in the region(s) sampled.
Blue lines: agreement between cluster and morphospecies number, orange lines: taxonomic accuracy. NT, Northern Territory, SA, South Australia, TAS, Tasmania, WA, Western Australia.
Table 2.
Agreement and taxonomic accuracy using clustering at a preset threshold of 3%, single threshold GMYC analysis and statistical parsimony.
Figure 4.
Geographical origin of samples and regional comparisons.
(A) Geographical origin of sequenced Australian individuals (green stars), red = specimens in paraphyletic clusters. (B) Molecular biodiversity estimation employed for regional comparison. Arrows = states compared; Numbers = number of clusters using 3% threshold for all samples from the two areas compared (N of clusters shared between two areas) % of clusters that perfectly agree with existing taxonomy; S = Sørensen Index.
Figure 5.
Relation between geographic distance and β-diversity (Sørensen index) for pairwise comparisons between localities.
Sorensen index for species numbers based on morphology (green), numbers of cox1 clusters, estimated at 3% threshold of genetic DNA distance (red) and derived from GMYC algorithm (orange).