Table 1.
Summary of Total Chaperone Genes Analyzed.
Table 2.
Summary of Uniformly Expressed Genes.
Figure 1.
Expression analysis of HSPA family members.
Gene expression levels of HSPA (HSP70) family members (rows) show little variability across 17 structures (columns, see text for abbreviations) of the adult mouse brain. Hspa1a is known to be an inducible heat shock gene.
Figure 2.
Expression analysis of DnaJ family members.
Expression levels of DnaJ (HSP40) subfamilies A, B, C, and Others (rows) are shown across 17 structures (columns) of the adult mouse brain. More than half of the genes exhibit some variability across the brain, but few have significant differences (≥2 expression quintiles in ≥2 structures). DnaJc7 (*) is also known as Ttc2, a tetratricopeptide repeat domain co-chaperone. Duplicated genes are listed where two data sets were available from the ABA (DnaJc29).
Figure 3.
Expression analysis of HSPC family members.
Gene expression levels of the HSPC (HSP90) chaperone family. All are near ubiquitously and highly expressed in the adult mouse brain.
Figure 4.
Expression analysis of TPR family members.
Expression levels of tetratricopeptide repeat (TPR) chaperones. The TPR chaperones are divided into four subfamilies, HSPC co-chaperones (* represents proteins identified through Picard lab website; see text), FK506 binding protein (Fkbp), Peptidylprolyl isomerase (Ppi), and Tetratricopeptide repeat domain (rows). Approximately half of all TPR genes show some variability across 17 brain structures (columns). N/D indicates no data was available for those specific regions. Duplicated genes are listed where two data sets were available from the ABA.
Figure 5.
Expression analysis of TPR family members.
Expression levels of tetratricopeptide repeat (TPR) chaperones. The TPR chaperones are divided into four subfamilies, HSPC co-chaperones (* represents proteins identified through Picard lab website; see text), FK506 binding protein (Fkbp), Peptidylprolyl isomerase (Ppi), and Tetratricopeptide repeat domain (rows). Approximately half of all TPR genes show some variability across 17 brain structures (columns). N/D indicates no data was available for those specific regions. Duplicated genes are listed where two data sets were available from the ABA.
Figure 6.
Expression analysis of HSPB family members.
The HSPB and Crystallin genes (listed in rows) generally show low levels of expression across structures of the adult mouse brain (columns). Duplicated genes are listed where two data sets were available from the ABA (Hspb8).
Figure 7.
Expression analysis of HSPH family members.
HSPH genes (rows) are ubiquitously and highly expressed across all 17 structures of the adult mouse brain (columns). The AAA+ ATPase family members exhibit a more variable pattern. N/D indicates no data was available for those specific regions and, duplicated genes are listed where two data sets were available from the ABA.
Figure 8.
Expression analysis of Chaperonin family members.
Expression levels of the chaperonin and HSPD/E genes. The two major families of the chaperonin genes are the Chaperonins and Prefoldins (rows). With a few exceptions, they exhibit a bimodal expression pattern across 17 structures of the adult mouse brain (columns). N/D indicates no data was available for those specific regions.
Figure 9.
Expression analysis of HSF family members.
HSF proteins generally show low levels of expression whereas HSF binding protein is highly expressed. Duplicated genes are listed where two data sets were available from the ABA (Hsf1).
Figure 10.
Expression analysis of other uncategorized chaperones.
Expression levels are shown of genes that function within the chaperone network but do not succinctly fit in the aforementioned families are categorized as ‘Other.’
Table 3.
Summary of Chaperone Gene Expression by Brain Region.
Table 4.
Summary of Chaperone Subcellular Localization.
Figure 11.
Summary of chaperone genes showing variable levels of expression.
The 30 genes that are variably expressed across ≥2 brain regions are shown. Genes from all groups except HSPC and HSF are represented. Duplicated genes shown are instances where the ABA had two data sets. Generally, variably expressed genes are higher in the forebrain and lower in the rest of the brain.
Figure 12.
Immunoblot analysis of DnaJb1, DnaJb2, and Tomm70a.
A. Immunoblots of different brain regions. Top Panel – A positive control sample for the antibody was loaded in the last late (labeled DnaJ1). Quantitation for DnaJb1, DnaJb2, and Tomm70a are shown in panels B–D, respectively.