Table 1.
Specificity of Human Monoclonal Fabs for Staphylococcal and Streptococcal Superantigens.
Figure 1.
Antibody detection of SEB in a complex antigen mixture by Western blot.
A 10–20% gradient SDS-PAGE separated crude S. aureus antigen preparations subsequently transferred onto nitrocellulose. Each Fab (1 µg/ml) was used to probe the nitrocellulose for 1 h. Lanes include: A- 50 ng purified SEB; B – 20 µg cell lysate; and C – 20 µg culture supernatant.
Table 2.
Kinetic Analysis of Immobilized Human Antibody Interactions with SEB in Solution.
Table 3.
Antibody Inhibition of Human T-Cell Responses to Bacterial Superantigens.
Figure 2.
Therapeutic capabilities of antibodies added after SEB in human PBMC cultures.
Fab or native full-length (FL) Mabs were used at a final concentration of 1000 nM and added at designated times after SEB (0.35 nM). Culture fluids were collected 16 h after SEB addition and IFNγ concentrations ascertained as described in the Materials and Methods. Panels A and C respectively show Fab and FL-Mab results. Antibody controls consisted of a SEA-specific Fab and SEB affinity-purified IgG from human sera (Panel B). Results are representative of two separate experiments.
Table 4.
Antibody Neutralization of SEB in vivo.