Figure 1.
Agarose gel images showing the effects of DMSO and betaine during the PCA assembly (A and C) and amplification (B and D) of IGF2R and BRAF gene fragments.
Based on a 20 µl reaction volume, both additives were introduced with increasing percentage (%) for DMSO, and molarity (M) for betaine; ‘No Additive’ lanes correspond to the control samples. A 1 kb DNA ladder in the outermost lanes marked at 400, 500 and 700 bp indicates the area of highest product band population. IGF2R and BRAF full-length gene fragments are shown at 517 bp and 512 bp, respectively.
Figure 2.
Agarose gel images showing the effects of DMSO and betaine during LCR assembly (A and C) and amplification (B and D) of IGF2R and BRAF gene fragments.
Based on a 20 µl reaction volume, both additives were introduced with increasing percentage (%) for DMSO, and molarity (M) for betaine; ‘No Additive’ lanes correspond to the control samples. A 1 kb DNA ladder in the outermost lanes marked at 400, 500 and 700 bp indicates the area of highest product band population. IGF2R and BRAF full-length gene fragments are shown at 517 bp and 512 bp, respectively.