Figure 1.
GFAP mRNA expression in mice after hypoxia-ischemia, assessed by quantitative rt-PCR.
Cortex from wild-type mice exposed to hypoxia-ischemia on P9, examined by quantitative rt-PCR immediately after (n = 6), 6 h (n = 4), 24 h (n = 3), 3d (n = 4), 7d (n = 4), and 21d (n = 4) after hypoxia-ischemia. The values represent a fold increase compared to 0 hours after HI. Data are mean ± SD (** p<0.01).
Figure 2.
Hemisphere and infarct volume in GFAP−/−Vim−/− and wild-type mice after hypoxia-ischemia.
Volume measurements in MAP-2 stained sections from GFAP−/−Vim−/− (GV) (n = 23) and wild-type (WT) (n = 19) mice at P12 and P31, i.e. 3 and 22 days after hypoxia-ischemia. (A) Ipsi- and contralateral hemisphere volume in GFAP−/−Vim−/− and wild-type mice. (B) Infarction volume in GFAP−/−Vim−/− and wild-type mice. Data are mean ± SD.
Figure 3.
Visualization of neuronal axons in the infact area and in the contralateral hemisphere.
Neuronal axons were visualized with antibodies against neurofilament proteins on sections from wild-type (A-D) and GFAP−/−Vim−/− (E-H) mice at P12, i.e. 3 days after hypoxia-ischemia. The frames indicate respective fields for higher magnification. Scale bar for A, C, E, G = 500 µm; for B, D, F, H = 50 µm.
Figure 4.
Quantification of NeuN+ and NeuN+BrdU+ cells at 22 days after hypoxia-ischemia.
(A) Number of NeuN+ cells per optical field in the ipsi- and contralateral subventricular zone, striatum and cortex, in GFAP−/−Vim−/− (GV) (n = 29) and wild-type (WT) (n = 28) mice at P31, i.e. 22 days after hypoxia-ischemia. (B) Number of NeuN+BrdU+ cells per optical field in ipsi- and contralateral subventricular zone, striatum and cortex, in GFAP−/−Vim−/− (n = 29) and wild-type (n = 28) mice at P31, i.e. 22 days after hypoxia-ischemia. The numbers represent cells counted on 8 µm thick stacks of confocal images within the 450 µm×300 µm optical fields in the respective brain regions. (C) Orthogonal sections showing NeuN+BrdU+ cells. Data are mean ± SD. (* p<0.05, ** p<0.01, *** p<0.001). Scale bar = 15 µm.
Figure 5.
Quantification of S100+ and S100+BrdU+ cells at 22 days after hypoxia-ischemia.
(A) Number of S100+ cells per optical field in ipsi- and contralateral subventricular zone, striatum and cortex, in GFAP−/−Vim−/− (GV) (n = 29) and wild-type (WT) (n = 28) mice at P31, i.e. 22 days after hypoxia-ischemia. (B) Number of S100+BrdU+ cells per optical field in ipsi and contralateral subventricular zone, striatum and cortex, in GFAP−/−Vim−/− (n = 29) and wild-type (n = 28) mice at P31, i.e. 22 days after hypoxia-ischemia. The numbers represent cells counted on 8 µm thick stacks of confocal images within the 450 µm×300 µm optical fields in the respective brain regions. (C) Orthogonal sections showing S100+BrdU+ cells. Data are mean ± SD. (* p<0.05, ** p<0.01). Scale bar = 15 µm.