Figure 1.
Effects of somatic cell ablation of β-catenin on germ cell apoptosis and entry into meiosis.
(A–B) Immunohistochemistry for germ cell marker TRA98 (red) and apoptotic marker cleaved caspase 3 (green) in control (SF1/cre;Ctnnb1f/+) and β-catenin conditional KO (cKO; SF1/cre;Ctnnb1f/−) ovaries at 17.5 dpc. The arrows indicate cells that are double-positive (yellow) for TRA98 and cleaved caspase 3. The inset in B is an image of a higher magnification of cells double-positive (yellow) for TRA98 and cleaved caspase 3. (C–F) Analysis of the meiotic status of germ cells via immunohistochemistry for SCP3 on germ cell smear obtained from the control and β-catenin conditional KO ovaries at 15.5 dpc. The samples were counterstained with nuclear DAPI (blue). Scale bar represent 100 µm in A–B and 10 µm in C–F.
Figure 2.
Effects of constitutively active form of β-catenin on female germ cell survival in the Wnt4 KO ovary.
(A–D) Whole mount light field images of Wnt4+/−;SF1/cre, Wnt4−/−;SF1/cre, Wnt4+/−;SF1/cre; Ctnnb1fl.(ex3), and Wnt4−/−;SF1/cre; Ctnnb1fl.(ex3) ovaries at birth. (E) Average total germ cell number was obtained from Wnt4+/−;SF1/cre, Wnt4−/−;SF1/cre, Wnt4+/−;SF1/cre; Ctnnb1fl.(ex3), and Wnt4−/−;SF1/cre; Ctnnb1fl.(ex3) ovaries at birth (n = 3 embryos for each genotype). Tukey tests revealed that the average germ cell number in Wnt4−/−;SF1/cre was significantly different from that in Wnt4+/−;SF1/cre (P = 0.017), Wnt4+/−;SF1/cre; Ctnnb1fl.(ex3) (P = 0.002), and Wnt4−/−;SF1/cre; Ctnnb1fl.(ex3) (P = 0.023). The asterisk represents statistical significance. (F–I) Immunohistochemical staining for androgen-producing enzyme CYP17 (green) was performed in Wnt4+/−;SF1/cre, Wnt4−/−;SF1/cre, Wnt4+/−;SF1/cre; Ctnnb1fl.(ex3), and Wnt4−/−;SF1/cre; Ctnnb1fl.(ex3) ovaries at birth. The inset in G represents a higher magnification of the CYP17-positive cells. Scale bar = 100 µm.
Figure 3.
Effects of flutamide treatment on development of the reproductive tract and germ cells in β-catenin conditional KO female.
(A–H) Whole mount images of reproductive tracts and ovaries and (I–L) immunohistochemistry for TRA98 were performed on the ovary of the control (SF1/cre;Ctnnb1f/+) and β-catenin cKO (SF1/cre;Ctnnb1f/−) female with or without flutamide treatment. o = ovary; arrow = epididymis, arrowhead = oviduct. Scale bar represents 500 µm in A–D and 100 µm in E–L.
Figure 4.
Involvement of Inhbb in germ cell loss in the absence of Wnt4.
(A–C) Whole mount in situ hybridization for Inhbb was performed on control ovary (SF1/cre;Ctnnbf/+ or Ctnnb1f/−) (A), β-catenin cKO ovary (SF1/cre;Ctnnbf/−) (B), and Wnt4−/−;SF1/cre; Ctnnb1fl.(ex3) ovary (C) at 13.5 dpc. (n = 2–3 for each genotypes). o = ovary, m = mesonephros. (D–F) Immunohistochemistry for TRA98 (red) and CYP17 (green) was performed on ovary sections from control (Wnt4+/−; Inhbb+/−), Wnt4 single KO and Wnt4; Inhbb double KO ovary at birth. (G) Light field microscopic images of the reproductive tract were taken from control female, Wnt4 single KO, and Wnt4; Inhbb double KO females at birth. Arrowheads indicate oviduct and arrows indicate epididymal structure. o = ovary. (J–L) Immunohistochemistry for TRA98 (red) and SCP3 (green) were performed on ovary sections from control, Wnt4 single KO and Wnt4; Inhbb double KO ovary at 15.5 dpc. At this stage, the female germ cells have not lost yet in the Wnt4 KO ovaries, allowing us to monitor the status of meiosis. The insets are the images of a higher magnification of cells double-positive (yellow) for TRA98 and SCP3. Scale bar represent 250 µm in A–C, G–I and 100 µm in D–F, J–L. (M) A proposed model for the somatic cell-derived pathway on female germ cell survival: In the mouse fetal ovary, WNT4 signals via β-catenin to decrease the expression of activin βB or Inhbb, which causes loss of meiotic germ cell. WNT4 also stimulates the production of follistatin (Fst), which acts to antagonize the activity of Inhbb. The WNT4/β-catenin pathway also prevents the ectopic production of androgens in the fetal ovary, which is not responsible for the germ cell loss. WNT4 could possibly regulate its own expression via β-catenin.