Figure 1.
Expression profiling and validation.
(A) Unsupervised hierarchical clustering by using 96 genes identified by class comparison as differentially expressed between relatively higher or lower MITF-expressing melanoma cell lines classified the cell lines into two main groups (Motif 1, green label; Motif 2, black label). (B) qPCR validation of selected targets in a subset of Motif 1 and 2 NZM cell lines. Unsupervised clustering of qPCR data confirmed the Motif 1 (green) and 2 (black) classifications. (C) Protein expression of MITF targets CDK2, BCL2, and MLANA agreed with MITF transcript levels and array and qPCR cell line stratification. *Motif 1 cell lines. (D) Immunofluorescence showed stronger staining for MITF and MLANA in the Motif 2 NZM06 cells compared to Motif 1 NZM09 cells.
Figure 2.
Motif 1 cell lines showed greater motility and migration in vitro.
(A) The number of Motif 1 cells (NZM09, NZM11, NZM22, NZM40, NZM52) that migrated through pored membranes in transwell (Boyden Chamber) assays was approximately 23-fold more than Motif 2 cell lines (NZM06, NZM12, NZM15, NZM42, NZM45; mean±SD is shown from the combined data of three separate experiments for each cell line; *** p<0.0001, t test). (B) Motif 1 cell lines NZM09 and NZM40 were significantly faster at wound repair in 2D scratch assays than Motif 2 NZM06 and NZM42 cell lines (mean±SEM; n = 3; *** p<0.001, two-way ANOVA). Representative movies of individual scratch assays for NZM09 and NZM42 are provided as supplementary Movie S1 and S2, respectively. (C) siRNA-mediated knockdown of MITF caused an almost 4-fold increase in migration of weakly invasive Motif 2 cell lines (NZM06, NZM15) in transwell assays compared to non-targeting siRNA controls (mean±SD from three separate experiments; *** p<0.0001, t test). MITF knockdown was confirmed by using qPCR and western blot (Figure S2).
Figure 3.
External validation with independent cell line data.
Unsupervised clustering by using our 96 gene invasion signature on the combined Zurich and Philadelphia (A), or Mannheim (B) cell line data of Hoek et al. [15] grouped cell lines into the cohorts of differing invasive potential originally identified by those authors, with Motif 1 corresponding to strongly invasive cohort C cell lines, and Motif 2 to weakly invasive cohort A cell lines.
Figure 4.
External validation with independent tissue data.
(A) Unsupervised analysis by using our filtered gene list (572 transcripts) on the tissue data of Haqq et al. [14] accurately classified skin, nevus, and melanoma tissue. MN, melanocytic nevus; PM, primary melanoma; MM, metastatic melanoma. (B) Application of our 96 gene invasion signature to the tissue data of Haqq et al. identified skin-like and nevus-like tumour samples representing Motif 1- and Motif 2-expressing tumour samples, respectively. Samples in red were derived from patients reported to have “type 1” tumours by the original authors.
Figure 5.
A model linking expression of lineage specification and extracellular sensing genes to invasive potential in melanoma.
Pathway analysis suggested that melanoma invasive potential may be mediated by the intersection of MITF-driven transcriptional networks with pathways involved in HIF/JUN activation and response to hypoxia.