Table 1.
Fecal VRE counts in different intestinal compartments of VRE colonized mice.
Table 2.
Total enterococcal and aerobic gram-negative count in fecal pellets.
Figure 1.
Comparable P. aeruginosa clearance from lungs in all treatment groups.
Three groups of mice were intranasally inoculated with P. aeruginosa after different pre-treatment schedules. Two groups of mice were treated with vancomycin for 19 days; in one of these VRE was administered by gastric inoculation (107 CFU) 5 days after the initiation of vancomycin treatment (VRE colonized mice), while in the other group no VRE was administered (Vancomycin controls). A third group of mice did not receive vancomycin or VRE (Naïve controls). Percentages of positive blood cultures at different time points are depicted below the graphic. Data are mean±SEM of 8 mice per group at each time point.
Figure 2.
Comparable increase in relative lung weight (mg whole lung/gram mouse) during P. aeruginosa pneumonia in all 3 groups.
Three groups of mice were intranasally inoculated with P. aeruginosa, after different pre-treatment schedules. Two groups of mice were treated with vancomycin for 19 days; in one of these VRE was administered by gastric inoculation (107 CFU) 5 days after the initiation of vancomycin treatment (VRE colonized mice), while in the other group no VRE was administered (Vancomycin controls). A third group of mice did not receive vancomycin or VRE (Naïve controls). Data are mean±SEM of 8 mice per group at each time point.
Figure 3.
Cytokine levels 6 hours after induction P. aeruginosa pneumonia.
TNF-α (A and D), IL-6 (B and E) and IL-10 (C and F) levels in lung and plasma of mice, 6 hours after intranasal inoculation with P. aeruginosa. Two groups of mice were treated with vancomycin for 19 days; in one of these VRE was administered by gastric inoculation (107 CFU) 5 days after the initiation of vancomycin treatment (VRE colonized mice), while in the other group no VRE was administered (Vancomycin controls). A third group of mice did not receive vancomycin or VRE (Naïve controls). Data are mean±SEM of 8 mice per group; * p<0.05 versus mice without vancomycin treatment or VRE colonization.