Table 1.
Sequenced clones isolated from selections along with their resistance profiles.
Figure 1.
Enriched genomic regions plotted along the P. aeruginosa PAO1 genome.
The genomic regions highlighted were were enriched during aminoglycoside resistance selections and identified via microarray. The aminoglycoside used in the selection and concentration are given in the labels of the concentric rings. The unselected library sample shows that signal was present all along the genome, indicating proper genomic coverage.
Table 2.
Common genes and genomic regions identified in selections.
Figure 2.
Hierarchical clustering of selection population microarray data, displaying most enriched genetic loci.
The selection populations are represented with different columns, with names given across the top. Enriched loci are shown in the rows. Regions colored in yellow are less enriched. Regions colored in blue are more enriched. Loci are listed as genomic position given in the center base pair of the locus, ORF number, or gene name. Colored boxes on the far right correspond to genomic regions identified in conventional sequencing and later confirmed to increase aminoglycoside Resistance. The following abbreviations were used to title arrays: A16 (amikacin 16 µg/ml population), A8 (amikacin 8 µg/ml population), G16 (gentamicin 16 µg/ml population), G8 (gentamicin 8 µg/ml population), T2 (tobramycin 2 µg/ml population), and Libr (unselected mixed libraries population).
Figure 3.
Contours of microarray signal intensity plotted according to genomic position for the genomic regions identified during sequencing, listed in Table 1.
The regions highlighted are those of (A) clone T2-C1, (B) clone T2-C3, (C) clone G16-C1, (D) clone G16-C2, and the two regions contained within clone A8-C1 (E and F). The Y-axis on each individual graph depicts the relative microarray signal intensity. The X-axis depicts the genomic position in base pairs. Below the X-axis are the ORFs for which the depicted genomic region encompasses.