Figure 1.
Flow chart of development of cucumber SSR markers.
Figure 2.
Cucumber linkage map developed from the present study.
The Bin names and genetic distances in cM are respectively listed on the left and right of the chromosomes. The number of SSR markers in each filled Bin is indicated in the boxes. White boxes indicated a recombination event with no markers. The short and long arms are indicated with S and L, respectively SDR = segregation distortion region.
Table 1.
Summary of the cucumber genetic map with RIL mapping population from the inter-subspecific cross between Gy14 and PI 183967.
Figure 3.
Integration of the seven linkage groups of cucumber with individual chromosomes.
(A1) Distribution of Type I/II (green) and Type III (red) repeats on cucumber chromosomes. (A2) DAPI staining was converted to black and white images. (A3) Localization of chromosome-specific fosmid clones on both arms of individual chromosomes, genetic location of arm-specific fosmid clones are indicated in Figure 2. (B) Localization of fosmids 4S (red) and 4L (green) together with Type III (red) and 45S rDNA (green) repeats on the mitotic chromosomes. Bar = 2.5 µm.
Figure 4.
An inversion detected by FISH on cucumber chromosome 5 between GY 14 and PI183967.
(A) Locations of the fosmid 12-7 (SSR20648, red) and 12-2 (SSR13340, green) on chromosome 5 of GY14. Red signals are located closer to the telomere than green signals. (B) Locations of the fosmid clones on chromosome 5 of PI 183967. Note that green signals are closer to the telomere. Bars = 3 µm.
Figure 5.
Distribution of PIC values for 642 SSR markers in cucumber.
PIC values were calculated from PCR among 11 cucumber inbred lines.
Table 2.
Cross-species transferability of 995 cucumber genomic SSR markers in melon, watermelon and pumpkin.