Table 1.
Frequency of homologous recombination in stressed S0 generation.
Table 2.
Frequency of homologous recombination in progeny of stressed plants of line 11.
Table 3.
Frequency of homologous recombination in progeny of stressed plants of line 1445.
Figure 1.
Expression of genes involved in homologous recombination in treated S0 and untreated S1 and S2 generations.
Relative expression after bleocin (A), paraquat (B), zebularine (C) and UV-C (D) treatment was measured by real-time PCR (with UBC28 as a reference gene not influenced by any treatment) as the amount of transcript in stressed S0 and non-stressed S1 and S2 progeny plants of line 1445 and normalized to the amount of transcript in corresponding mock-treated S0, S1 and S2 generations, respectively. RAD51, BRCA1 and MIM were strongly up-regulated in S0 by bleocin, zebularine and UV-C and, to a weaker but significant degree, by paraquat. Up-regulation after UV-C is apparent only at the second time point since plant material was harvested immediately after irradiation. ATM showed only minor and mostly non-significant changes in expression after stress. None of the tested genes showed an increased amount of transcript in the non-stressed S1 and S2 progeny.