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Figure 1.

Flow of participants through the Pennington Phase 1 CALERIE trial.

The CONSORT diagram was published previously [6].

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Table 1.

Physical characteristics of 48 men and women in weight maintenance at baseline.

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Figure 2.

Experimental design (A) and body weight and composition changes (B) at the completion of the study.

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Table 2.

Total daily energy expenditure (TDEE; kcal/day) measured by doubly labeled water at M0, M3 and M6.

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Figure 3.

The effect of caloric restriction on AREE (change in TDEE at M3 and M6 after adjusting for SMR (a measure of sedentary energy expenditure).

* represents a significant change from baseline.

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Figure 4.

The effect of caloric restriction (CR, n = 12) on all components of daily energy expenditure (Top Panel).

Total daily energy expenditure (TDEE) is measured by doubly labeled water over a 2-week period whereas sedentary 24-h energy expenditure (24h-EE) is measured in a respiratory chamber. Spontaneous physical activity was calculated as previously reported [7] whereas the thermic effect of food (TEF) was calculated in the chamber as per Tataranni et al [38] and the same percentage of energy intake (9–12% across subjects) was assumed to assess the extra TEF in free living conditions. The changes in total daily energy expenditure after 3 and 6 months of CR (Bottom Panel) are shown and those representing a metabolic adaptation (larger than due to weight loss) are highlighted in grey.

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