Table 1.
New oligo-nucleotide primers designed in this study.
Figure 1.
Study profile of CSF specimens with results.
Figure 2.
Multiplex PCR schemes and their coverage for Pneumococcal serotypes of Bangladesh
Figure 3.
Amplified serotype (1, 2, 14 & 18) genome from culture negative CSF specimens by 1st reaction of multiplex PCR scheme.
Lane 1–10 & 14–17: Culture negative latex Positive CSF, Lane 11–13: Culture negative ICT Positive CSF, Lane 18: Positive Control Containing amplified cps gene and genome of 4 known isolates of reaction 1, L 19: Reagent control–reagents devoid template, L 20: 100bp ladder.
Figure 4.
Distribution of capsular types among A) Isolates and B) Culture negative meningitis cases.