Figure 1.
Study design.
Table 1.
Decrease levels of selected transcripts after bacterial death.
Table 2.
Ratios between decrease levels of persistent and labile transcripts.
Figure 2.
Real time RT-PCR results analyzed by using TMev software.
24 hours after heat-killing, RNA extraction and random reverse transcription, the transcripts were analyzed by real time PCR and fold changes were calculated between T 0h positive controls and heat-treated samples. Fold changes results were analyzed by TMev software. A) Comparison transcripts decay profile 24 hours after lethal heat-treatment of P. aeruginosa (PA) and E. coli (EC) in non-stored (NS) and previously 3 weeks stored in mineral water (S) conditions. B) Comparison of results analysis of P. aeruginosa with or without calculation of ratios with one of the most persistent transcript Ct value. NSPA = Non-stored P. aeruginosa; SPA = Stored P. aeruginosa; NSEC = Non-stored E. coli; SEC = Stored E. coli.
Table 3.
Genes analyzed in this study and corresponding function.