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Figure 1.

Single frames from high speed video recordings of spore discharge in four coprophilous fungi.

a, Ascobolus immersus, with 8 ascospores discharged from ascus tip, 60 µs into the launch. b, Podospora anserina, with 4 ascospores harnessed by mucilaginous appendages, 96 µs after release from apex of fruiting body at left of frame. c, Pilobolus kleinii, sporangium with sap trailing behind, 0.8 ms after beginning of discharge. Undischarged sporangium at top of frame. d, Basidiobolus ranarum, single spore carrying portion of dehisced conidiophore, 24 µs into the launch. Scale bars, a, b, d, 50 µm, c, 1 mm. Frame rates a, 100,000 fps, b, 250,000 fps, c, 50,000 fps, d, 210,000 fps.

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Table 1.

Ballistics of spore and sporangium discharge in four coprophilous fungi based upon high speed video analyses and different models for the effects of viscous drag on particle flight.

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Figure 2.

Predicted trajectories of spores and sporangia of four fungi based on launch data obtained by high speed video microscopy.

Trajectories of spores and sporangia of Ascobolus immersus (A.i., blue), Podospora anserina (P.a., red), Basidiobolus ranarum (B.r., green), and Pilobolus kleinii (P.k., blue in inset). Points indicate projectile positions at 10 ms intervals. The truncated trajectories of A. immersus, P. anserina, and B. ranarum are indicative of the dominance of viscous forces over inertial forces in the motional regimes for these launches. Inertia is more significant for the flight of the larger sporangia of P. kleinii. Launch angles of 40° (A.i.), 30° (P.a., P.k.), and 20° (B.r.), were chosen to separate the trajectories from one another but also reflect the phototropic orientation of these asci, sporangiophores, and conidiophores in nature.

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