Table 1.
Hybrid Sperm Motility in sons of mothers from the High and Low D. mojavensis lines and in the intercross populations of the High and Low lines, when females crossed to D. arizonae fathers.
Figure 1.
Design used to produce the recombinant D. mojavensis females to be mated to inbred D. arizonae males.
Figure 2.
Composite interval mapping results for intraspecific variation for interspecific hybrid male sterility.
Likelihood ratio values for the F2 (dashed red), RF2 (dashed blue), and combined (solid black) data sets are given for the X through 5th chromosome. The X-chromosome scale is normalized to “combined” map length for the F2 and RF2 maps. The threshold for significance is the horizontal line (red- F2, blue- RF2, and black- combined). (a) Mapping results considering each son as measure of the mother's phenotype. Black asterisks indicate the location of markers with significant pairwise epistatic effects and yellow asterisks indicate two additional markers showing a significant deviation from the additive expectation. There is clear evidence for a QTL of major effect on the 3rd chromosome for all data sets, on the 2nd chromosome for the F2 and combined data sets, and on the 5th for the F2 dataset. (b) Mapping results of the BLUP values for each mother. There is evidence for a QTL on major effect on the 3rd chromosome in all three datasets and on the 2nd chromosome in the combined dataset.
Table 2.
Maximum Likelihood Position and Effect Size for individual son and BLUP QTL found in Composite Interval Mapping.
Table 3.
Significant Marker-Marker Interactions
Figure 3.
Interaction effect between marker genotype and cross-type on motility score.
The average motility score of the homozygous marker genotype on the second chromosome (dmoj2210-High or Low parental line) relative to which cross it was derived (F2 or RF2). There is a significant interaction between marker and cross type.