Table 1.
Sensitivity test on 279 published insect pre-miRNAs.
Table 2.
Distributions and optimal ranges of quantifiable features of pre-miRNAs.
Table 3.
miRNAs expressed in B. moria.
Figure 1.
Predicted stem-loop structures of precursors and mature forms (highlight in red) of newly-identified miRNAs based on our direct cloning approach from the silkworm.
Hairpins longer than 120 nt were truncated; these hairpins are indicated by a double slash preceding the stem. Scaffold numbers and genomic positions are indicated in parentheses.
Figure 2.
Expression confirmation of novel miRNAs identified by cloning from specific development stages of silkworm using stem-loop RT PCR.
For bmo-miR-2008, we detected its three different mature segments, bmo-miR-2008a, bmo-miR-2008b and bmo-miR-2008c; for bmo-miR-2009, we detected only the mature segment from 5′ arm of its precursor. U6 snRNA serves as a positive control.
Figure 3.
Length heterogeneity is found in the 5′ and/or 3′ end, the cloned sequences (C) of bmomiR-1, bmo-miR-10, bmo-miR-13, bmo-miR-31, bmo-miR-71, bmo-miR-77, bmo-miR-263a, bmo-miR-275 and bmo-miR-317 have nucleotides difference (highlighted in red) in 5′ and/or 3′ ends of the sequences as compared with their predicted sequences (P) or homologs among closely related species.
Figure 4.
Three different segments of cloned bmo-miR-2008 (highlight in red, blue and green respectively) derived from the 5′ arm of its predicted pre-miRNA S147.
Scaffold number and genomic positions are indicated in parentheses.
Figure 5.
Expression profiles of 15 mature miRNAs in 14 developmental stages.
Data from each miRNA were showed as dendrograms indicating expression correlation among genes. Samples and miRNAs are displayed in rows and columns, respectively. The relative expression values ranged from +5 log10 to −5 log10.
Table 4.
High-probability targets of 15 silkworm miRNAs.