Figure 1.
Late flowers from LS1 (B, C), LS3 (D), early flowers from LS1 (E), LS3 (F) and wild type Micro-Tina (A).
These are late flowers that set parthenocarpic fruits (C and D). There were only 1–3 such flowers in each mutant. All other flowers are small and never develop large petals although they do have enlarged sepals. Vascular veins on the petals show a different pattern than the wild type as well. G–H: LS1 (left and center) and wild type (right) fruits. A shoot with flower buds grows out of one fruit (right). I–J: A control mutant showing slightly enlarged sepals.
Figure 2.
PCR amplified T-DNA right border/plant junction bands using genome walk procedure [Siebert et al. 1995].
Lanes A-LS1/DraI; B-LS1/EcoRV; C- LS3/StuI. Each numbered band is cloned and sequenced. Bands 1 and 2 are identical so only sequence from 2 is used. But 3 and 4 are different, probably representing two different insertions in LS3. Sizes of molecular weight marker are indicated to the left.
Figure 3.
Insertion sequence from LS1, LS2 and a control plant.
Sequences in red for LS1 and LS3 (band 4) are PCR primers designed to detect the insertion sequence in wild type tomato genome. Underlined sequences are those that match tomato genomic DNA in GenBank and are used to generate data for Table 1.
Table 1.
Number of insertion sequence matchs in the 12 tomato chromosomes.
Figure 4.
VENN diagram showing the number of cDNAs that exhibit a two-fold change in expression in LS1 and LS3 mutants compared to wild-type plants.
The down-regulated cDNAs are diagrammed in Panel A and the up-regulated cDNAs are diagrammed in Panel B. In both diagrams, the area of overlap indicates the number of cDNAs which are similarly regulated in both LS1 and LS3 mutants.
Figure 5.
MA plots of microarray data from LS1 and LS3 tomato mutants before and after loess normalization.
Table 2.
Up- and down-regulated flower-related genes in both LS1 and LS3 mutants in tomato.*