Figure 1.
Photomicrographs showing phosphorilated Histone H3 (HH3, a proliferating mitotic marker) within the dentate gyrus of Non Tg mice.
A–B: Single labelling of HH3 positive cells (arrows) in the dentate gyrus of 2 (A) and 12 months (B) Non Tg mice. C–D: Dual labeling of HH3 positive cells (arrows) and glial cells (GFAP, blue) in the dentate gyrus of 2 (C) and 12 months (D) Non Tg mice. E–F: Bar graphs showing the area density of HH3 positive cells within the dorsal dentate gyrus (all layers included) of Non-Tg males (E) and females (F) mice. GCL: Granular Cell Layer, ML: Molecular Layer. ** = p<0.01 compared to 2 months; *** = p<0.001 compared to 2 months;•• = p<0.01 compared to 3 months; ## = p<0.01 compared to 2 and 3 months; ### = p<0.001 compared to 3 months; ◊ = p<0.05 compared to 4 months; ◊◊ = p<0.01 compared to 4 months; ∧ = p<0.05 compared to 4 and 6 months; + = p<0.05 compared to 2, 3 and 6 months.
Figure 2.
Brightfield micrographs showing HH3 labelled cells within the dentate gyrus of 3xTg-AD e.
A–B: Dual labeling of HH3 positive cells (arrows) and glial cells (GFAP, red) in the dentate gyrus of 2 (A) and 12 months (B) 3xTg-AD mice. C–D: Bar graphs showing the area density of HH3 positive cells within the dorsal dentate gyrus (all layers included) of 3xTg-AD males (C) and females (D) mice. GCL: Granular Cell Layer, ML: Molecular Layer. ** = p<0.01 compared to 3 months; *** = p<0.001 compared to 3 months; • = p<0.05 compared to 2 months •• = p<0.001 compared to 2 months; ## = p<0.01 compared to 2 and 3 months; ◊ = p<0.05 compared to 4 months; ∧ = p<0.05 compared to 2 and 4 months.
Figure 3.
Bar graphs showing the mean area density HH3 labelled cells within the GCL of the dentate gyrus of both 3xTg-AD and control nonTg-AD mice.
A–B: Males (A) and females (B) dorsal GCL. C–D: Males (C) and females (D) dorsal GCL. Asterisks indicate a significant difference in the means.
Figure 4.
Photomicrographs showing the presence of β-amyloid within the pyramidal neurones of CA1 as well as the presence of a plaque in 12 months 3xTg-AD mice (B) compared to a nonTg control animal (A).
C–D: Bar graphs showing the number of cells containing β-amyloidin the hippocampal CA1 of males (C) and females (D) 3xTg-AD mice. E–F: Linear correlations between the mean number of cells containing β-amyloid in the hippocampal CA1 and the mean area density of HH3 positive cells in the GCL of the dentate gyrus of males (E) and females (F) 3xTg-AD mice. In G we can see the accumulation phosphorilated Tau within the CA1 of a 3xTg-AD mice. Or: CA1 Stratum Oriens, Py: CA1 stratum Pyramidale, Rad: CA1 Stratum Radiatum, LMol: CA1 Stratum Lacunosum Moleculare. * = p<0.05 compared to 2 months; ** = p<0.01 compared to 2 months; ∧ = p<0.05 compared to 3 months.