Figure 1.
X-ray diffraction and plant ontogeny.
A) Synthetic crystalline cellulose (Avicel) displayed a diffractogram pattern consistent with diffractogram from plant biomass derived from Arabidopsis whole plant biomass (B) Schematic of the sample scan used to estimate the experimental accuracy of pressed biomass at 22.5° 2-theta. Experimental accuracy was determined to be 8.99% (C) Roots, leaves and shoots from Arabidopsis plants were sub-sampled and analyzed by XRD showing variability of RCI between tissues. (D) RCI values for ball milled samples, whole plant sample and stem, rosette (leaf) and root values. *Rosette RCI was significantly lower than stems or roots (P<0.001 ANOVA) whereas stems and roots were not significantly different. (E) RCI values for ball milled samples, whole plant sample and stem, rosette (leaf) and root values with experimental accuracy included.
Table 1.
Plant species analyzed within this study.
Figure 2.
RCI values and their hierarchical cluster dendogram for a foliar samples from diverse range of species indicates a large degree of variability.
A) Samples were clustered based on values for their RCI (B).
Figure 3.
Hypocotyls lengths and RCI of etiolated pea (Pistum sativum) seedlings compared with light grown seedlings were significantly different.
(A) Histogram of hypocotyl length for light grown and (B) dark grown. Dark grown hypocotyls had a significantly lower RCI that light grown (C) (*P<0.001 ANOVA). (D) Arabidopsis plants expressing YFP::CESA6 displayed a transverse orientation under dark conditions and longitudinal array under light conditions, images are time averages of 61 frames taken 10 sec apart for 10 min. Maximal linear trajectories particles was 14.66 mm for dark and 26.8 mm for light grown. Scale bar = 10 mm (E) FTIR spectral variance plot of light versus dark grown pea plant samples.
Figure 4.
Pairwise comparison of foliar traits with RCI (A) RCI versus leaf mass per unit area B) RCI versus leaf length.