Figure 1.
Analysis of differentially expressed genes: a) Venn diagrams: * indicates significant overlaps (p<106); b) Heatmaps of the 595 differentially regulated genes in both M vs. N1 and M vs. N2 (left) and the 418 differentially regulated genes in both M vs. N1 and N2 vs. N1; c) Heatmap of 15 genes in the intersection of all three groups (gene symbols listed on the right). In heatmaps, rows represent genes and columns represent samples from N1, N2, and M groups. Genes are clustered using row-normalized signals and mapped to the [−1,1] interval (shown in scales beneath each heatmap). Red and green represent high and low expression values, respectively.
Table 1.
Gene Ontology Categories
Figure 2.
The analysis has been performed for >1200 predefined datasets using GSEA 2.0 software. Signal values for each gene are obtained by collapsing the probe values using max_probe algorithm. Representative datasets, significantly enriched (FDR<50%, or NPV< = 0.01) between any two groups and corresponding heatmaps (depicting relative gene expression changes of core enrichment) are shown in a) N2 vs. N1 and b) M vs. N1. Datasets that are enriched in both the original and validation analyses are marked with *. c) Heatmaps of ribosomal proteins and ubiquitin mediated proteolysis illustrate transitional trends in gene expression across the N1, N2 and M groups.
Table 2.
Demographics