Table 1.
Data pertaining to 118 patients with RRP were subjected to further analysis.
Table 2.
Contingency table of HPV type versus nature of clinical course.
Table 3.
Contingency table of HPV type versus four individual criteria that taken together constituted the composite “aggressiveness” label.
Figure 1.
Papilloma biopsies were taken at the time of enrollment and subjected to HPV typing.
For those cases where the enrollment surgery was not their first surgery, the interval in days since the last surgery was recorded. Variables were transformed so that they would comply with a Gaussian distribution. The natural log of the number of days since the last surgery are depicted on the y axis. The left panel depicts a linear regression against the square root of the age at the time of enrollment. The model predicts that a 4 year old last had surgery 69 days (2.3 months) ago and a 9 year old last had surgery 113 days (3.7 months) but only 20% of the variability is accounted for by this model. The right panel depicts a boxplot of the same data but clustered by HPV type. The geometric mean of the number of days since the last surgery was 119 days (3.9 months) for those with HPV 6 and a similar 97 days (3.2 months) for those with HPV 11. The t-test p-value was 0.25.
Table 4.
Listing of literature in which HPV typing was reported.
Figure 2.
Agarose gel electrophoresis of HPV 6 and 11 specific PCR fragments.
The first 2 lanes after the ladder (both labeled “1”) represent the wells for HPV 6 positive control clone for the TS-PCR using either primer pair 6930–6932 (designed to be specific for HPV 6) or 6931–6933 (designed to be specific for HPV 11). The same pattern applies to the remaining lane couplets. The couplet labeled “2” represents HPV 11 positive control clone. Couplets 3–8 are RRP specimens and couplet 9 is the PCR negative control. In this gel we see that that laryngeal DNA used in couplet 3 contained only HPV 11 and the remainder contained only HPV 6.
Figure 3.
HPV 6 and 11 genotyping by restriction fragment length polymorphism.
BstN I enzyme is a single cutter for the 929 bp HPV6 and 11 consensus PCR fragment. HPV6 possesses the enzyme recognition site thus the digestion yields two fragments of size 526 and 403 bp while HPV11 does not. Lanes 1 and 2 are HPV 6 and 11 positive controls. Lanes 3–13 are RRP patients.