Figure 1.
Vero and MRC-5 cell growth on Cytodex 1 microcarriers in serum-free cultures.
Microscopic observation shows (A) Vero and (B) MRC-5 cells on microcarriers in M-VSFM after 3 days culture.
Table 1.
The maximum virus titers for four-serotype DEN virus strains and the cloned DNA-derived DEN-4 viruses in Vero cells and MRC-5 cells on microcarriers, in serum-containing and serum-free media.
Table 2.
Genomic sequences of the cloned DNA-derived DEN-4 viruses in Vero and MRC-5 cells on microcarriers, in serum-containing and serum-free media.
Table 3.
Nucleotide and amino acid mutations in the WE1-W02R (E-NS1) genetic fragment of the cloned DNA-derived DEN-4 viruses propagated in Vero and MRC-5 cells at three consecutive passages using serum-free medium.
Table 4.
Nucleotide and amino acid mutations in the W28-W29R (NS3) genetic fragment of the cloned DNA-derived DEN-4 viruses propagated in Vero and MRC-5 cells at three consecutive passages using serum-free medium.
Table 5.
Nucleotide and amino acid mutations in the W21-W23R (NS4B-NS5) genetic fragment of the cloned DNA-derived DEN-4 viruses propagated in Vero and MRC-5 cells at three consecutive passages using serum-free medium.
Table 6.
The number of amino acid mutations and the mean diversity for the three genetic fragments of the cloned DNA-derived DEN-4 viruses propagated in Vero and MRC-5 cells in serum-free cultures.