Figure 1.
EndoS has glycosidase activity on all four human IgG subclasses.
Panel A. Glycan structure of human IgG. Glycan on the γ−chains of IgG attached to aspargine 297. GlcNAc, N-acetylglucosamine; Fuc, fucose; Man, mannose; Gal, galactose; NeuAc, sialic acid. Cleavage site for EndoS and recognation site for Lens culinaris agglutinin lectin (LCA) are indicated. Panel B. Purified IgG 1–4 incubated with EndoS and analyzed by SDS-PAGE and stained. Panel C. IgG 1–4 incubated with EndoS and analyzed using LCA lectin blot.
Figure 2.
Hydrolysis of human IgG-subclasses by EndoS in plasma environment.
Human plasma treated with EndoS or PBS and followed by IgG glycan hydrolysis detection using LCA lectin ELISA of the purified IgG fraction. The results are presented as percent hydrolysis of each subclass compared to signals from untreated plasma. Means and standard deviations, indicated with error bars, were calculated from three independed experiments using blood from three different donors.
Figure 3.
EndoS(E235Q) binds to all IgG-subclasses.
Panel A. Slot-blot representing the binding of EndoS and EndoS (E235Q) to each IgG subclass immobilized onto a nitrocellulose membrane in amounts: 3, 1.5 and 0.75 µg. The binding was detected using antiserum against EndoS. Panel B. Binding of EndoS (E235Q) to immobilized IgG-classes using BIAcore technology. The selected plot shows EndoS (E235Q) binding to IgG1 using hydrolysed IgG1 as a reference (bulk changes subtracted).
Table 1.
Kinetic constants of EndoS binding to immobilized human IgG1–IgG4.
Figure 4.
EndoS treatment of IgG subclasses inhibits binding of IgG to FcγRII.
Panel A. Binding of purified IgG subclasses, with or without EndoS treatment, to FcγRIIa and FcγRIIb immobilized to a microtitter plate. HRP-labeled protein G was used for detection of bound IgG subclasses. (−) indicates intact IgG and (+) EndoS hydrolysed IgG. Means, standard deviations (indicated with error bars), and p values (calculated using Student's t-test) were determined from three separate experiments. Panel B. Binding of IgG subclasses to immobilized receptors as visualized using BIAcore surface plasmon resonance. Plot shows a typical sensorgram, here the IgG1 binding to FcγRIIa. An empty flow cell is used as reference (subtracted).
Table 2.
Kinetic constants of IgG1–IgG4 binding to different Fc-receptors.
Figure 5.
EndoS treated IgG does not bind to FcγRIIa on K562 cells. Panel A.
The relative binding of radioactive IgG, With or without EndoS treatment, to K562 cells. The cells were incubated with 125iodine-labelled IgG (intact or EndoS-treated). The radioactivity of the washed cell pellets was detected. The binding of 125I- IgG (intact) to K562 cells, presented here as 100%, represents a specific IgG binding to K562 cells that could be inhibited by addition of cold IgG. Means, standard deviations (indicated with error bars), and p values (calculated using Student's t-test) were determined from three separate experiments. Panel B. K562 cells were incubated with human plasma treated with EndoS or PBS. The cells were resuspended in lysis buffer and analyzed by SDS-PAGE and Western blot using antiserum against human IgG.
Figure 6.
EndoS treated IgG does not bind to monocytes. Panel A.
Monocytes were incubated with 125iodine-labelled IgG (intact or EndoS-treated). After incubation for 30 minutes at room temperature, the proteins from cell lysates, 10 µg total protein, were separated by 10% SDS-PAGE. The gel was dried and analyzed by phosphorimaging. Panel B. Flow cytometry analysis showing the decreased binding of IgG to activated monocytes in blood treated with EndoS. Human blood was treated with EndoS before addition of leukocyte activator fMLP. The IgG binding to monocytes was detected using mouse anti-human IgG and FITC-labelled goat anti-mouse IgG as a secondary antibody.
Figure 7.
Dissociation of IgG from FcγRII upon treatment with EndoS. Panel A.
IgG bound to K562 cells dissociates from FcγRIIa upon incubation with EndoS but not with EndoS(E235Q). K562 cells were incubated with plasma and subsequently with EndoS, EndoS(E235Q) or PBS. Cell lysates, 10 µg total protein, were analysed for IgG by SDS-PAGE and blot using antiserum against human IgG. Panel B. IgG bound to monocytes dissociates from FcγRs after treatment with EndoS. Monocytes were incubated with plasma and later with EndoS or PBS. Resuspended cell pellets were analyzed for IgG by blot using antiserum against human IgG. The glycan of IgG was detected by blot and reactivity with LCA lectin. Panel C. A BIAcore setup showing EndoS affecting the IgG1 dissociation from an immobilized receptor FcγRIIa. In two parallel experiments, the injection of EndoS (black curve) is compared to the injection of buffer (broken line) at the same time-point during the dissociation phase of the IgG1- FcγRIIa interaction.