Figure 1.
The PAS effect of phage ΦMFP on E. coli MFP on Luria-Bertani agar plates.
Only disks containing the β-lactam antibiotics aztreonam and cefixime (indicated by “+” symbols) produced large phage plaques in their proximity. Gentamicin and tetracycline gave no PAS effect. This host strain was resistant to both amoxicillin and trimethoprim/sulfamethoxazole. Note the absence of phages on the left-hand control plate indicating the lack of prophage induction.
Figure 2.
Plaque sizes of phage ΦMFP on E. coli MFP with and without 50 ng/mL of cefotaxime (CTX) in Luria agar plates.
Figure 3.
Increase in phage titer in the presence of the cephalosporin cefotaxime (CTX).
E. coli strain MFP was infected with phage ΦMFP in Luria liquid medium supplemented at the time of infection with 20 ng/mL of cefotaxime, or left untreated. The multiplicity of infection was ∼5. Chloroform was added at various times after infection to lyse the infected cells.
Figure 4.
Plaque sizes of various coliphages with and without cefotaxime (CTX) in Luria agar plates.
Phages RB32 and RB33 were grown on E. coli strain MFP (50 ng/mL CTX) at 37°C; and T4, T3 and T7 were grown on E. coli strain AS19 (30 ng/mL CTX) at 25°C. All plaques were photographed at identical magnifications.
Figure 5.
The PAS effect of phage T4 on various E. coli SOS and filamentation mutant strains.
T4 was grown on E. coli sulA-inactivated and lexA non-inducible mutant strains (defective SOS systems), as well as an ftsZ-inactivated mutant strain (non-antibiotic induced filamentation), in the presence of disks of cefotaxime (CTX). Isogenic wild-type strains (wt) are also included and representative plaques demonstrating the PAS effect are indicated by red arrows. All plates were photographed at identical magnifications.