Figure 1.
The Fas2 landmark system. A1) Dorsal view of a maximal projection showing longitudinal and transverse Fas2 immunoreactive fascicles. A2) Transversal view at the height of neuromere a3. B1) Idealized dorsal scheme B2) Idealized transverse scheme. Arrows in A1 and B1 show the neuromere a9 (“terminal plexus”). Scale bars: 50 µm in A), 25 µm in B).
Figure 2.
Mapping of AST-A-IR neurons in whole-mount preparations of the thoracic and abdominal neuromeres of L3 larva in the Fas2 landmark system. A) Dorsal view. B) Transversal view at the height of neuromere a1/a2. C) Idealized dorsal scheme. D) Idealized transverse scheme. Scale bars: 50 µm in A), 25 µm in B). Immunostaining is shown in green, Fas2 in magenta.
Figure 3.
Morphology of CAPA- and HUGIN neurons.
Mapping of PRXa-IR neurons in whole-mount preparations of the thoracic and abdominal neuromeres of L3 larva in the Fas2 landmark system. A) Dorsal view. B) Transversal view at the height of neuromere a3. C) Idealized dorsal scheme. D) Idealized transverse scheme. Scale bars: 50 µm in A), 25 µm in B). Immunostaining is shown in green, Fas2 in magenta.
Figure 4.
Mapping of Ccap-GAL4xUAS-cd8.gfp expressing neurons in whole-mount preparations of the thoracic and abdominal neuromeres of L3 larva in the Fas2 landmark system. A) Dorsal view. B) Transversal view at the height of neuromere a3. C) Idealized dorsal scheme. D) Idealized transverse scheme. Scale bars: 50 µm in A), 25 µm in B). Marker protein expression is shown in green, Fas2 in magenta.
Figure 5.
Morphology of corazonin neurons.
Mapping of corazonin-IR neurons in whole-mount preparations of the thoracic and abdominal neuromeres of L3 larva in the Fas2 landmark system. A) Ventral view. B) Transversal view at the height of neuromere a6. C) Idealized dorsal scheme. D) Idealized transverse scheme. Scale bars: 50 µm in A), 25 µm in B). Immunostaining is shown in green, Fas2 in magenta.
Figure 6.
Morphology of eclosion hormone neurons.
Mapping of eh-GAL4xUAS-cd8.gfp expressing neurons in whole-mount preparations of the thoracic and abdominal neuromeres of L3 larva in the Fas2 landmark system. A1) Idealized dorsal scheme. A2) Idealized transverse scheme.
Figure 7.
Morphology of FMRFamide neurons.
Mapping of FMRFa-IR neurons in whole-mount preparations of the thoracic and abdominal neuromeres of L3 larva in the Fas2 landmark system. A) Dorsal view. B) Transversal view at the height of neuromere t2. C) Idealized dorsal scheme. D) Idealized transverse scheme. Scale bars: 50 µm in A), 25 µm in B). Immunostaining is shown in green, Fas2 in magenta.
Figure 8.
Morphology of IFamide neurons.
Mapping of IFa-IR neurons in whole-mount preparations of the thoracic and abdominal neuromeres of L3 larva in the Fas2 landmark system. A) Dorsal view. B) Transversal view at the height of neuromere a3. C) Idealized dorsal scheme. D) Idealized transverse scheme. Scale bars: 50 µm in A), 25 µm in B). Immunostaining is shown in green, Fas2 in magenta.
Figure 9.
Morphology of leucokinin neurons.
Mapping of leucokinin-IR neurons in whole-mount preparations of the thoracic and abdominal neuromeres of L3 larva in the FasII landmark system. A) Dorsal view. B) Transversal view at the height of neuromere a3. C) Idealized dorsal scheme. D) Idealized transverse scheme. Scale bars: 50 µm in A), 25 µm in B). Immunostaining is shown in green, Fas2 in magenta.
Figure 10.
Mapping of Pea-MIP-IR neurons in whole-mount preparations of the thoracic and abdominal neuromeres of L3 larva in the Fas2 landmark system. A) Dorsal view. B) Transversal view at the height of neuromere a2. C) Idealized dorsal scheme. D) Idealized transverse scheme. Scale bars: 50 µm in A), 25 µm in B). Immunostaining is shown in green, Fas2 in magenta.
Figure 11.
Mapping of pdf-GAL4xUAS-cd8.gfp expressing neurons in whole-mount preparations of the thoracic and abdominal neuromeres of L3 larva in the Fas2 landmark system. A) Dorsal view of the posterior part of the VNC. B) Transversal view at the height of neuromere a8. C) Idealized dorsal scheme. D) Idealized transverse scheme. Scale bars: 50 µm in A), 25 µm in B). Marker protein expression is shown in green, Fas2 in magenta.
Figure 12.
Morphology of tachykinin-related peptide-expressing neurons.
Mapping of TRP-IR neurons in whole-mount preparations of the thoracic and abdominal neuromeres of L3 larva in the FasII landmark system. A) Dorsal view. B) Transversal view at the height of neuromere a1. C) Idealized dorsal scheme. D) Idealized transverse scheme. Scale bars: 50 µm in A), 25 µm in B. Immunostaining is shown in green, Fas2 in magenta.
Figure 13.
Distribution of c929-GAL4 driven expression of synaptic markers.
A) Dorsal view of the VNC of a c929-GAL4xUAS-GFP larva, voltex projection. B) Lateral view of a8–9 of a c929GAL4xUAS-GFP larva, voltex projection. C) Dorsal view of a8–9 of a c929GAL4xUAS-GFP larva, voltex projection. Note the reduced morphology of the neuropil in a9 ( = the terminal plexus), immunolabeled against the synaptic marker protein synapsin (arrow) in A–C). D) Dorsal view of a c929GAL4xUAS-RDL.HA larva, maximum projection. RDL.HA immunostaining labels the cell bodies, descending neurites (asterisks) and the terminal plexus (arrow). Scale bars: 100 µm in A), 20 µm in B–C), 50 µm in D). HA-immunostaining or GFP expression is shown in green, synapsin-IR in magenta.
Table 1.
Employed fly lines
Table 2.
Employed antisera
Table 3.
Assignment of putative main compartment identities as suggested by morphology, immunolabeling intensities and distribution of synaptic markers