Figure 1.
Phylogenetic distribution across land plants of included taxa and PCR success of tested loci.
The cladogram indicates the major land plant lineages [34]–[35]. The lineages sampled in this study are highlighted in yellow. The success of each colored-coded primer in amplifying at least one species is indicated for each of the lineages; open white boxes indicate primer failure in all taxa tested; white boxes with an “X” indicate missing sample.
Figure 2.
Properties of nine plant loci tested as putative barcodes.
Blue bars indicate PCR success; yellow bars indicate percent success in differentiating between species of a pair; maroon bars indicate PCR success combined with the ability to differentiate between species of a pair.
Table 1.
Comparison of results for nine individual loci tested as putative barcodes on 46–48 species pairs of land plants.
Table 2.
Wilcoxon Signed rank tests of divergence among loci.
Figure 3.
Properties of two-locus pairs tested as putative barcodes.
Only those locus pairs with PCR success greater than 90% are included. Yellow bars indicate percent success in differentiating between species of a pair only; maroon bars indicate PCR success combined with the ability to differentiate between species of a pair.
Table 3.
GenBank BLASTn results of trnH-psbA and rbcL-a as a barcode tested singly and as a pair.
Table 4.
Comparisons of results for pairs of two loci for trnH-psbA, rpoB, rpoC, rbcL-a, matK, and ITS tested in all combinations as putative barcodes on 48 species pairs of land plants.