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Expression and purification of the mammalian translocator protein for structural studies

Fig 4

Stable HEK293 cell line expressing a cleavable TSPO.

Comparison of the best clones of bovine and pig TSPO using FSEC. Normalised to total protein concentration with Bradford assay, determined using the cell lysates. Pig TSPO shows a 2-fold higher expression compared to bovine TSPO and was therefore chosen for subsequent large-scale expression.

Fig 4