Temporal transcriptomic profiling of pulmonary thromboembolism reveals persistent NETosis- and ferroptosis-associated gene signatures and enhanced thrombolysis with adjunctive DNase I
Fig 7
Additional pharmacological validation of the NETs-TLR9-NF-kB/GPX4 axis in the rabbit PTE model.
Serum levels of (A) MPO-DNA, (B) TLR9, (C) phospho-p65, (D) total p65, (E) phospho-p65/total p65 ratio, and (F) GPX4 were measured by ELISA. Relative mRNA expression of (G) TLR9 and (H) GPX4 was measured by qRT-PCR. Data are presented as mean ± SD with all individual data points shown; n = 5 biologically independent animals per group. One-way ANOVA followed by Dunnett’s multiple-comparisons test was used, with the Model group as the reference. * P < 0.05; ** P < 0.01; *** P < 0.001 versus Model.