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Estrogen deficiency induces pelvic floor muscle atrophy via ERα/GLUT4 pathway

Fig 1

Estrogen deficiency induces PFM atrophy associated with ERα downregulation in OVX rats.

(A) Serum estrogen levels at 12 weeks after surgery. (B) Body weight monitoring from 5 to 12 weeks after surgery. (C) H&E staining results of the PFM of rats in the OVX and Sham groups. (D, E) MASSON staining results of the PFM of rats in the OVX and Sham groups. (F) Distribution of myofiber CSA in the PFM of rats in the OVX and Sham groups. (G, H) Fast (red) and slow (green) muscle staining results of the PFM of rats in the OVX and Sham groups. (I, J) Immunofluorescence results for ERα (red) and ERβ (green) of the PFM of rats in the OVX and Sham groups. Data are presented as mean ± SD. *p < 0.05, **p < 0.01, ns, no significance.

Fig 1

doi: https://doi.org/10.1371/journal.pone.0349371.g001