Age-related upregulation of p16 expression in mouse ovarian somatic cells correlated with reproductive function decline p16 expression and ovarian aging in mice
Fig 5
Age-dependent decline in ovulation with preserved fertilization capacity.
Mice were treated with hCG to induce ovulation, followed by in vitro fertilization (n = 10–15 / group). This figure shows fertility parameters in young (4–5 weeks of age), 30, 45, and 60 weeks of age mice: (A) Number of ovulated oocytes after hCG injection. (B) Degeneration rate of ovulated oocytes (%). (C) Fertilization rate (2-cell stage/MII oocytes). (D) Embryo development rate from 2-cell stage to 4-cell stage (4 cells/2 cells); (E) Development rate from 2-cell stage to 8-cell stage (8 cells/2 cells); (F) Development rate to morula stage (morula/2 cells); (G) Development rate to blastocyst stage (BL/2 cells) BL: blastocyst. wks: weeks. Data are presented as mean ± SEM and analyzed using one-way ANOVA followed by Tukey#39;s post-hoc test (*p < 0.05 compared to young control).