S-palmitoylation regulates the function of the mitochondria-associated endoplasmic reticulum membrane to alleviate the senescence of nucleus pulposus cells
Fig 5
Detection of protein expression levels and observation of cellular microstructure.
(a) Percoll centrifugation to isolate subcellular structural MAMs, and Western Blot to detect GRP75, VDAC1, Drp1, and Mfn2 protein expression levels. ***p < 0.001. (b) TEM observation of cell microscopic morphology.