Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19
Fig 7
Poldip2 depletion variably affects cytokine expression after SARS-CoV-2 infection.
Male and female hACE2/Poldip2 mice received a nasal instillation of PBS or SARS-CoV-2. BAL fluid and lungs were collected after 7 days and processed as in Figure 6 to measure the expression of cytokine mRNAs in lung tissue (A) and proteins in BAL (B). Bars represent means ± SEM of data from n = 5-19 animals. Basal protein levels in +/ + PBS were 1.7 ± 0.1 pg/ml (TNFα), 5.0 ± 0.3 pg/ml (IL-1β), 11.8 ± 0.8 pg/ml (IL-6), 4.2 ± 0.5 pg/ml (IFN-γ). Data were analyzed using 2-way ANOVA: ns, not significant; * P < 0.05; ** P < 0.01; *** P < 0.001.