Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19
Fig 6
Poldip2 depletion tends to attenuate chemokine expression.
Male and female hACE2/Poldip2 mice were euthanized 7 days after intranasal administration of PBS or SARS-CoV-2. Bronchoalveloar lavage (BAL) fluid was collected before harvesting lungs for RNA extraction as in Figure 3. mRNAs were measured by RT-qPCR in lung tissue (A) and protein ELISAs were carried out in BAL (B). Bars represent means ± SEM of data from n = 4-17 animals. Basal protein levels in +/ + PBS were 40 ± 20 pg/ml (MCP1) and 111 ± 31 pg/ml (CXCL1). Data were analyzed using 2-way ANOVA: ns, not significant; ** P < 0.01; *** P < 0.001.