Poldip2 deficiency attenuates lung disease severity in a mouse model of COVID-19
Fig 5
Poldip2 depletion reduces neutrophil infiltration in lung after SARS-CoV-2 infection.
Lungs from male and female hACE2/Poldip2 mice were harvested 7 days after nasal PBS or virus instillation and processed for immunofluorescence. Tissue sections were stained to detect the leukocyte common antigen (CD45, red), the neutrophil marker myeloperoxidase (MPO, green) and nuclei (blue), as shown in representative micrographs (left). Cells positive for both CD45 and MPO were counted in 10-13 images per animal (right). Bars represent means ± SEM of data from n = 4-6 mice. Data were analyzed using 2-way ANOVA: ns, not significant; *P < 0.05, **P < 0.01.