Unraveling the role of ChREBP in lung adenocarcinoma: Expression, regulatory networks, and potential functional impact
Fig 5
Alteration of expression levels of selected genes in dnChREBP-overexpressing NCI-H1975 cells.
(A-D) RNA-seq results. Bar graphs depict the Log2 FC in genes enriched in the extracellular space GO term (A), genes enriched in the Hallmark EMT gene set (B), hub genes (C), and ChREBP target genes (D). (E) Gene expression analysis using RT-qPCR was conducted on selected genes to validate the RNA-seq findings. Gene expression levels were normalized against the three most stable HKGs, RPL13a, ACTB, and B2M. Asterisks (*) denote statistically significant differences (p < 0.05) compared to the expression levels in control cells.