Comparative genomics-based development of a LAMP assay for rapid and reliable in-field detection of Fusarium oxysporum f. sp. cubense tropical race 4
Fig 4
Sensitivity of the Cand-23-LAMP system as assessed in plasmid dilutions (a) and in plasmid-spiked plant tissue extractions (b).
TTR: Time To Result (mins:ss). The colour and shape of the data points indicates the DNA volume used in the reaction; blue triangles (5 µL) or yellow squares (1 µL) for TTR means, while replicates are shown as solid circles. Error bars indicate the Standard Error (SE) of the mean. The horizontal black line indicates the threshold above which a sample is considered negative, and the dashed red horizontal line indicates the value assigned to the samples that produced null signal (negative). The asterisks on top indicate whether the mean is positive with a 95% of confidence level.