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Antitumoral immunity induced by gel ethanol ablation to treat unresectable colorectal cancer metastases in the liver

Fig 6

Increased infiltrating cytotoxic T-cells in the contralateral tumor after ablative treatments.

Representative IHC-stained contralateral tumors for CD8a treated by (A) sham, (B) EC-ethanol, (C) RFA, or (D) combination (EC-ethanol + RFA) at 7 days post-ablation. Scale bars = 400 µm. Quantification and comparison of the amount of CD8a + CTLs within (E) the entirety of the contralateral tumor and (F) within the margins of the tumor periphery (n = 5-6). Error bars are standard error of the mean (SEM). *P < 0.10, **P < 0.05, ***P < 0.01, ****P < 0.001.

Fig 6

doi: https://doi.org/10.1371/journal.pone.0347625.g006