Antitumoral immunity induced by gel ethanol ablation to treat unresectable colorectal cancer metastases in the liver
Fig 5
Substantial accumulation of cytotoxic T-cells around the margins of the ablation zone.
Closeups of the EC-ethanol ablation zone margins after (A) 3 days (black box) and (B) 7 days (red box), with the margins (region denoted with black arrow and boundary delineated with yellow and green dashed lines) defined as 400 µm out from the ablation zone perimeter. Quantification and comparison of the amount of CD8a + CTLs within the margins at (C) 3 and (D) 7 days post-ablation (n = 3-5). Error bars are standard error of the mean (SEM). Scale bars = 300 µm. *P < 0.10, **P < 0.05, ***P < 0.01, ****P < 0.001.