Identification of an optimal exogenous gene insertion site (P–M) and establishment of a reverse genetics system for aMPV/A
Fig 3
Comparison of EGFP expression levels in aMPV/A-EGFP recombinant virus.
Detection of EGFP expression in Vero cells inoculated with seven aMPV/A-EGFP recombinant viruses at 48 hpi (MOI = 0.1). A–G represent fluorescence micrographs of Vero cells infected with raMPV/A-NP-EGFP (A), raMPV/A-PM-EGFP (B), raMPV/A-MF-EGFP (C), raMPV/A-FM2-EGFP (D), raMPV/A-M2SH-EGFP (E), raMPV/A-SHG-EGFP (F), and raMPV/A-GL-EGFP (G), respectively. Scale bar = 100 μm. H is the quantitative analysis of relative EGFP fluorescence intensity in infected cells; the x-axis shows different recombinant virus strains, and the y-axis represents relative fluorescence intensity (no unit). Fluorescence intensity was quantified using ImageJ software, and data are presented as the mean values of at least three independent experiments. P < 0.05 indicates a statistically significant difference in fluorescence intensity compared with other recombinant strains.