Identification of an optimal exogenous gene insertion site (P–M) and establishment of a reverse genetics system for aMPV/A
Fig 2
The growth curve of the aMPV/A-EGFP recombinant virus.
Growth kinetics analysis of the parental aMPV/A strain and seven EGFP-expressing recombinant aMPV/A strains in Vero cells. The x-axis represents hours post-inoculation (hpi) with the virus, and the y-axis indicates viral titers expressed as log₁₀ 50% tissue culture infective dose per milliliter (log₁₀ TCID₅₀/mL). Vero cells were inoculated with each virus at a multiplicity of infection (MOI) of 0.1, and viral titers were determined at 4-hour intervals using the Reed-Muench method. Data are presented as the mean values of at least three independent experiments. P < 0.05 indicates a statistically significant difference compared with other recombinant strains.