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An optimized protocol for metabolic measurement in 3D tumor spheroids derived from primary and established glioblastoma cells

Fig 6

Results of different normalization approaches and comparison to 2D.

(A) and (B) are graphs of the Pearson correlation coefficient of the raw or normalized OCR of basal (A) or maximal respiration (B) values with the cell number for 2D and 3D spheroids measurements. C) and D) are graphs of the relative variance of the raw or normalized OCR of basal (A) or maximal respiration (B) values for the 2D and 3D spheroids measurements.

Fig 6

doi: https://doi.org/10.1371/journal.pone.0347569.g006