An optimized protocol for metabolic measurement in 3D tumor spheroids derived from primary and established glioblastoma cells
Fig 5
(A) t-SNE clustering of spheroids from the four cell lines, showing the distinct response of LN229 compared to the other GBM cell types. Each point represents a single spheroid. Each point corresponds to the data of a single spheroid. (B) Illustration of the energetic state of the four GBM spheroid types at baseline and after FCCP injection to achieve maximal respiration. Error bars represent the standard error of the mean (SEM). The following number of spheroids was used for each group: nGBM10k = 42, nGBM4 = 44, nLN229 = 47, nU138 = 48.