Deciphering the molecular network of Trichostatin A in regulating Alzheimer’s disease screening of core genes and mechanistic investigation based on multidimensional bioinformatics and molecular simulation
Fig 2
Schematic of TSA target prediction and AD-related gene screening.
(A). 2D chemical structure of TSA (PubChem CID: 444732). (B). Venn diagram of TSA potential targets predicted by ChEMBL, PharmMapper and SwissTargetPrediction, with 949 non-redundant unique targets obtained. (C). PCA plots of merged training cohort (n = 295) before/after ComBat batch correction. (D). Volcano plot of AD DEGs (screening thresholds: |logFC| > 0.4, adj.P.Val < 0.05). (E). Heatmap of module-trait correlations from WGCNA. (F). Bar chart of gene counts in each WGCNA co-expression module. (G). Venn diagram of the intersection between 1363 DEGs and 2756 genes in the WGCNA MEturquoise module (1070 overlapping genes obtained). (H). Venn diagram of the intersection between 1070 module genes and 949 TSA targets (60 key genes screened).