Advanced machine learning-guided optimization platform for high-yield soluble expression of Pseudomonas aeruginosa exotoxin A in engineered Escherichia coli strains
Fig 2
Impact of temperature and redox additives on ETA solubility.
(A) Soluble ETA yield across four induction temperatures in SHuffle T7. Linear regression line shown with 95% confidence band. (B) Effect of redox additives on soluble yield at 12°C. Data are mean ± SD (n = 3). Different letters indicate statistically significant differences (p < 0.05, one-way ANOVA with Tukey’s post-hoc test).