MobileLAMP: A low-cost, portable incubation device for isothermal nucleic acid amplification
Fig 10
Detection of SARS-CoV-2 target using colorimetric RT-LAMP using MobileLAMP compared with thermocycler.
In positive LAMP reactions, the pH indicator dye changes from pink (negative) to yellow (positive) due to acid production during DNA amplification. The detection was carried out at 602 copies/μL (second row), 6022 copies/μL (third row) and 60221 copies/μL (fourth row) compared to NTC (no template control, first row). The figure shows results before incubation (left column, all pink) and after incubation, with duplicate samples tested in both thermocycler (middle two columns) and MobileLAMP (right two columns). MobileLAMP successfully amplified the targets as indicated by colour change from pink to yellow, demonstrating equivalent performance to the commercial thermocycler. Note: For colour-blind accessibility, besides the distinct hue and brightness shift from dark pink to lighter yellow, positive reactions appear in the ‘After incubation’ columns for both devices at all target concentrations, while the NTC (first row) remains pink in all conditions.