DJ-1 alleviates high glucose-induced podocyte injury via activating ERK1/2 signaling
Fig 2
DJ-1 attenuates the high glucose-induced apoptosis of HPCs.
(A) Representative Western blot images and quantitative statistical bar graphs of protein expression levels of DJ-1 in HPCs treated with mannitol (MG) or D-glucose were analyzed by Western blotting analysis and Image J software, respectively. (B) The mRNA levels of DJ-1 in HPCs treated with MG or D-glucose were analyzed by qRT-PCR analysis. (C) The cell viability was measured by CCK-8 assays in HPCs treated with MG, LG, HG and HG + DJ-1, respectively. (D) The cell viability was analyzed by EdU assays in HPCs treated with MG, LG, HG and HG + DJ-1, respectively. (E) The cell apoptosis was analyzed by Annexin V-FITC/PI apoptosis assay in HPCs treated with MG, LG, HG and HG + DJ-1, respectively. (F) The apoptotic rate of HPCs treated with MG, LG, HG and HG + DJ-1 was analyzed by GraphPad Prism 8 software. (G) Representative Western blot images and quantitative statistical bar graphs of protein expression levels of cleaved caspase 3 and Bcl2 in HPCs treated with LG, HG and HG + DJ-1 were analyzed by Western blotting analysis and Image J software, respectively. MG: mannitol, LG: 5.5mM D-glucose, MG: 15mM D-glucose, HG: 30mM D-glucose, DJ-1: transfected with 1 μg/mL DJ-1-Flag-pcDNA3.1 plasmid. Data are shown as mean ± SD and representative of three independent experiments. *p < 0.05; **p < 0.01; ***p < 0.001.