Bioinformatics analysis and qRT-PCR validation of iron metabolism-related genes in pediatric asthma
Fig 13
qRT-PCR validation of biomarkers in clinical samples.
(A-D) Relative mRNA expression levels of C19orf12, GDF15, XK and IREB2 were measured in peripheral blood samples obtained from patients with PA (n = 12) and Controls (n = 8). Data are presented as mean ± standard error of mean of relative quantification values calculated using the 2^ − ΔΔCt method with β-actin as the internal reference. Statistical significance was assessed by unpaired Student’s t-test. *p < 0.05, **p < 0.01, ***p < 0.001.