Bioinformatics analysis and qRT-PCR validation of iron metabolism-related genes in pediatric asthma
Fig 6
(A). Gene set enrichment analysis (GSEA) mountain map presentation of biological functions in the integrated Gene Expression Omnibus (GEO) datasets (combined datasets). (B–E). GSEA results showed significant enrichment for all genes in the following pathways: Stambolsky Targets of Mutated TP53 DN (B), Rutella Response to CSF2RB and IL4 DN (C), TGF β signaling pathway (D), and Hinata NFKB targets fibroblast up (E). In the mountain map, the color represents the adjusted p-value (adj.p-value): darker red indicates a smaller adj.p-value, and darker blue indicates a larger adj.p-value. In the bubble plot, the bubble size represents the gene set size, and the bubble color indicates the adj.p-value, with darker red corresponding to smaller values and darker blue corresponding to larger values. Red in the heatmap denotes high expression, whereas blue denotes low expression. GSEA screening criteria were false discovery rate (FDR) value (q-value) <0.25 and adj.p-value <0.05, with Benjamini-Hochberg (BH) as the p-value correction method.